Probiotics are live micro-organisms with edible safety and beneficial to human health. A γ-aminobutyric acid (GABA) producing strain was isolated from yogurt samples. Based on 16S rDNA sequence, phylogenetic analysis, morphological and physiological characterization, the strain was identified as Lactobacillus brevi, which belongs to the category of probiotics. The yield of GABA reached 2.03 g/L after optimization of culture conditions and media components. Ultraviolet irradiation was used to treat the original strain. The accumulation of GABA was 2.31 g/L, which was increased 11.4% compared with that of original strain. The mutant GABA-producing strain was stable after several generations. These results showed that the application field of probiotic products could be broadened.
ZOU Jianxun, MAO Mao, ZHANG Lining, LI Huidan, LOU Xudong, CHEN Weiqing
. Study on γ-aminobutyric acid-producing probiotics[J]. Bulletin of Fermentation Science and Technology, 2015
, 44(1)
: 22
-27
.
DOI: 10.16774/j.cnki.issn.1674-2214.2015.01.003