A mutant strain with high L-tryptophan productivity obtained by&nbsp;Atmospheric and Room Temperature Plasma

YANG Lipeng, LI Xiaogang, WEI Aiying, CHEN Jinlong,MA Fengyong, JIA Huiping, MENG Gang

doi:10.16774/j.cnki.issn.1674-2214.2015.01.009

Bulletin of Fermentation Science and Technology >

2015 , Vol. 44 >Issue 1: 28 - 32

DOI: https://doi.org/10.16774/j.cnki.issn.1674-2214.2015.01.009

A mutant strain with high L-tryptophan productivity obtained by Atmospheric and Room Temperature Plasma

Expand

Ningxia EPPEN Biotech Co., Ltd., Yinchuan 750100, China)

Fold

Abstract

In this study, a tryptophan high-yielding mutant TRP-YP-3-2 was obtained by Atmospheric and Room Temperature Plasma (ARTP) mutation technique. Strain TRP-YP-3-2 showed faster growth rate, greater glucose consumption rate and higher yield of tryptophan compared with the original strain (TRP-1201). The yield of L-tryptophan produced by TRP-YP-3-2 reached 61.4 g/L and the conversion rate of glucose reached 19.25%, which were increased by 15.41% and
22.77% respectively compared with the original strain. Study on the genetic stability of tryptophan production showed that TRP-YP-3-2 possessed a good genetic stability after 30 generations of culture. The tryptophan high-yielding mutant had great economic value, and the breeding method
established in this study could provide reference for other industrial microbial mutation breeding.

Key words： Atmospheric and Room Temperature Plasma (ARTP); tryptophan; high throughput screening

Cite this article

YANG Lipeng, LI Xiaogang, WEI Aiying, CHEN Jinlong,MA Fengyong, JIA Huiping, MENG Gang . A mutant strain with high L-tryptophan productivity obtained by Atmospheric and Room Temperature Plasma[J]. Bulletin of Fermentation Science and Technology, 2015 , 44(1) : 28 -32 . DOI: 10.16774/j.cnki.issn.1674-2214.2015.01.009

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article