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Bulletin of Fermentation Science and Technology >

2015 , Vol. 44 >Issue 2: 7 - 11

DOI: https://doi.org/10.16774/j.cnki.issn.1674-2214.2015.02.008

Screening and biotransformation optimization of a strain catalyzing 2-hydroxy-4-(methylthio) butyronitrile to methionine hydroxy analogue

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  • Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China
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Abstract

ZJB12048, a nitrilase-producing genetic engineering strain, was isolated. Under the optimized transformation conditions as followed, reaction temperature 50 ° C, initial pH value 7.0 (100 mmol/L citric acid/sodium citrate buffer solution), concentration of wet cells 0.02 g/mL and the concentration of substrates 150 mmol/L, 2 -hydroxy -4 -(methylthio) butyronitrile were transferred into methionine hydroxy analogue by the rested cells. And the obtained product was identified
as 2-hydroxy-4-(methylthio) butyric acid using HPLC, LC-MS and 1H NMR.

Key words： screening; 2-hydroxy-4-(methylthio) butyronitrile; 2-hydroxy-4-(methylthio) butyricacid; nitrilase; identification

Cite this article

LI Zongtong, JIN Liqun . Screening and biotransformation optimization of a strain catalyzing 2-hydroxy-4-(methylthio) butyronitrile to methionine hydroxy analogue[J]. Bulletin of Fermentation Science and Technology, 2015 , 44(2) : 7 -11 . DOI: 10.16774/j.cnki.issn.1674-2214.2015.02.008

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