Cytidine Diphosphate Choline (CDPC) is an effective pharmaceutical ingredient with broad market prospect. Currently, yeast fermentation is the most advanced production method for CDPC in China, which has advantages of less consumption of culture, high conversion rate and good product quality. The process of perfusion culture can separate the fermentation products continuously based on material circulation and tangential filtration. In order to solve the disadvantage
of limited production capacity and low efficiency of equipment utilization, a new perfusion system was applied in CDPC biosynthesis. The results showed that the production capacity was 3.96 times higher compare with that of non-perfusion method and the color degree of CDPC solution (A430) reduced
by 77.7%. The application of perfusion method in CDPC production effectively improved the product quality, production capacity and equipment utilization, and meanwhile significantly reduced
the production cost.