Catalytic reaction of lipase in organic phase is an important branch of non-aqueous enzymatic catalysis. In this paper, the catalysis of lipase in organic phase and the key factors are reviewed. It also comprehensively described the immobilization of lipase and its immobilization methods. Meanwhile, the applications of lipase in organic phase are summarized, such as fatty acid ester synthesis, food and pharmaceutical industry and new energy generation. In the last part, the
further study and application of lipase in organic phase are prospected.