The nisA and nisI gene were amplified through PCR technique using genomic DNA from Lactococcus lactis as template. The nisA gene was ligated to pMG36e vector and the circular plasmid pMG36e-nisA containing nisA gene was constructed. The nisI gene was later ligated to pMG36e-nisA plasmid to obtain the recombinant plasmid of pMG36e-nisA-nisI. The recombinant plasmid pMG36e -nisA -nisI was then transformed to L. lactis and a positive colony AI was successfully obtained. The result of nisin fermentation by the genetically engineered strain AI indicated that its yield increased by 20.07% compared with the original strain. AI was confirmed as a stable, high-yield nisin producing strain.