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Bulletin of Fermentation Science and Technology >

2015 , Vol. 44 >Issue 4: 24 - 29

DOI: https://doi.org/10.16774/j.cnki.issn.1674-2214.2015.04.008

Identification of psychrophilic bacteria in raw milk from Hangzhou area based on 16S rDNA sequences analysis and BIOLOG method

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  • 1. School of Biological and Chemical Engineering/School of Light Industry, Zhejiang University of Science and Technology,Hangzhou 310023, China; 
    2. Zhejiang Provincial Key Lab for Chemical and Biological Processing Technology of Farm Products,Hangzhou 310023, China; 
    3. Zhejiang Provincial Collaborative Innovation Center of Agricultural Biological ResourcesBiochemical Manufacturing, Hangzhou 310023, China;
    4. State Key Laboratory Cultivation Base and Key Laboratory of VisionScience, Wenzhou Medical University, Wenzhou 325027, China
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Abstract

Based on raw milk provided by four pastures in Hangzhou district, 15 psychrophile were isolated from culture plate and their growth curves were obtained. According to morphology, 16S rDNA sequences analysis and BIOLOG method, the isolated strains were identified as Pseudomonas sp., Pseudomonas synxantha, Pseudomonas fluorescens and Lactobacillus sp.. Among them, there are 13 species of Pseudomonas sp., which are the dominant bacteria in raw milk from Hangzhou.

Key words： raw milk; psychrophile microorganisms; BIOLOG identifieation; 16S rDNA sequence

Cite this article

YE Hui，XIE Haihua, HUANG Jun, LIU Shiwang, MAO Jianwei, LOU Jian . Identification of psychrophilic bacteria in raw milk from Hangzhou area based on 16S rDNA sequences analysis and BIOLOG method[J]. Bulletin of Fermentation Science and Technology, 2015 , 44(4) : 24 -29 . DOI: 10.16774/j.cnki.issn.1674-2214.2015.04.008

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