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Bulletin of Fermentation Science and Technology >

2015 , Vol. 44 >Issue 4: 48 - 50

DOI: https://doi.org/10.16774/j.cnki.issn.1674-2214.2015.04.013

Determination of mycophenolic acid related substances by HPLC and optimization of pH of mobile phrase

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  • 1. Fujian Hequan Biotechnology Co., Ltd., Jianyang 354200, China;
    2. Fujian Province Jianyang Wuyi MSG Co., Ltd., Jianyang 354200, China
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Abstract

Mycophenolic acid related substance was analyzed by HPLC equipped with Kromasil C18 chromatogram column (4.6 mm×200 mm, 5 μm×100A) and the reasonable pH of mobile phase was determined. The same sample was analyzed for 6 times and determined according to area normalization method. The optimal pH of mobile phrase was determined on the basis of relative standard deviation (RSD%) of mycophenolic acid percentage. The method showed good stability, high sensitivity and good reproducibility when the pH value of the mobile phrase was 4.5.

Key words： HPLC; mycophenolic acid; related substances; pH of mobile phase

Cite this article

ZHANG Hongfei, LIN Jinnan, LIAN Zilong, WENG Yangwei, YANG Bin . Determination of mycophenolic acid related substances by HPLC and optimization of pH of mobile phrase[J]. Bulletin of Fermentation Science and Technology, 2015 , 44(4) : 48 -50 . DOI: 10.16774/j.cnki.issn.1674-2214.2015.04.013

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