Pilot-scale microbial fermentation is based on the small scale experiment in the lab，which is carried out under simulated industrial production conditions． To ensure the possibility for the future manufacture process，the research and the manufacture process should maintain consistency． The aim of the pilot of microbial fermentation is to verify and recheck the reaction conditions of the process obtained from small scale，providing the guidance for the industrial production． As the link between fundamental research and industrial production，pilot of microbial fermentation is not only necessary to the product development but also provides meaningful experiences and data support for industrial production． Moreover，it is an effective way to reduce the risk in realizing industrialization． Pilot of microbial fermentation is influenced by many factors． Thus，it is of great significance to study and utilize these factors．