A bacterial strain Pseudomonas putida ZJPH1606 containing carbonyl reductase was screened out from soil，which can reduce 2'-( Trifluoromethyl ) acetophenone to ( R) -1-［2-( trifluoromethyl ) phenyl］ethanol with enantiomeric excess ( e． e． ) higher than 99． 0%． By single factor experiment， glucose，beef extract and MgSO4·7H2O were found to be significant factors influencing the carbonyl reductase production． In order to improve the enzyme production，the medium components were further optimized by response surface methodology． The results showed that the enzyme activity reached highest when the fermentation medium was composed of glucose 38． 5 g /L，beef extract 32． 7 g /L，MgSO4·7H2O 1． 1 g /L． Under the optimum conditions，the enzyme activity reached 0． 31 U/g，increased by 71． 0% compared to the original medium components． This study enriched the gene sequences database of carbonyl reductase and provided a new approach for the efficient synthesis of ( R) -1-［2-( trifluoromethyl phenyl］ethanol．