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Effect of arginine on L-tryptophan production by Escherichia coli

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  • 1.National and Local United Engineering Lab of Metabolic Control Fermentation Technology,Tianjin 300457,China;
    2.Tianjin Engineering Lab of Efficient and Green Amino Acid Manufacture,Tianjin 300457,China;
    3.College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China

Abstract

L-tryptophan,one of the essential amino acids for human and animals,is widely used in
the food,feed stuff and pharmaceutical industries.L-tryptophan producing strain Escherichia
coli TRTH was used as original strain in this article and sodium arginine was added to the
medium to decrease the accumulation of by-products and increase the yield of desired product.
The effects of sodium arginine on L-tryptophan fermentation were investigated in a 30 L
fermentor.The results indicated that when added with 0.2g/L sodium arginine,the biomass,Ltryptophan
 production and glucose conversion rate were 41.5g/L,34.5g/L and 18.0%,
respectively,increased by 5.46%,5.83% and 2.86% compared with that without sodium
arginine addition,and the accumulation of acetate was decreased by 10.99%.

Cite this article

YANG Mengchen,LIU Zhenyu,CHEN Ning,XU Qingyang . Effect of arginine on L-tryptophan production by Escherichia coli[J]. Bulletin of Fermentation Science and Technology, 2017 , 46(1) : 16 -19 . DOI: 10.16774/j.cnki.issn.1674-2214.2017.01.005

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