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Effects of IPTG addition time on fermentation of α-ketobutyric acid in Escherichia coli

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  • 1.College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China;
    2.National and Local United Engineering Lab of Metabolic Control Fermentation Technology,Tianjin 300457,China

Abstract

The study investigated the effect of IPTG addition time on theα-ketobutyric acid   producing strain Escherichia coli THRD/pWSK29-ilvA in the aspects of cell growth,   productivity,byproducts formation and sugar consumption.The results indicated that when   IPTG was supplemented after the OD600reached 10to 15,the biomass reached as high as 6.5g/L with the titer ofα-ketobutyric acid 16g/L.The glucose/acid conversion ratio was the highest   compared to other groups with different IPTG addition time,and the acetic acid concentration   decreased significantly.The optimized IPTG induction time was applied in a 7.5Lfermentor,
and the results showed that the titer ofα-ketobutyric acid reached 22g/L,higher than previously
reported results.

Cite this article

LI Juan,QI Junsheng,WANG Ting,MAO Qian,HAN Chao,LI Yanjun . Effects of IPTG addition time on fermentation of α-ketobutyric acid in Escherichia coli[J]. Bulletin of Fermentation Science and Technology, 2017 , 46(2) : 77 -82 . DOI: 10.16774/j.cnki.issn.1674-2214.2017.02.003

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