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Research on novel cell viability EZMTT assay

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  • Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals,Zhejiang University of Technology,Hangzhou 310014,China

Abstract

This study focused on the application of EZMTT assay based on tetrazolium salts/formazans reaction for cell viability,as well as its superiority evaluation.The accuracy of  EZMTT assay was evaluated on efficiency and toxicity.When the cell number is 3 000to 500 000, the linear dose response can be achieved within 4hours.The EZMTT assay exhibited higher  sensibility and effectiveness compared with WST-8assay in cell viability test.Besides,the low  toxicity allows EZMTT track cell growth in long-term,and the EZMTT reaction could be   stopped by 0.06%SDS or 50μmol/L HCL.The high efficiency and low toxicity demonstrated  the great potential of EZMTT assay for cell viability test.

Cite this article

ZHANG Jingjing,RUAN Benfang . Research on novel cell viability EZMTT assay[J]. Bulletin of Fermentation Science and Technology, 2017 , 46(2) : 88 -91+106 . DOI: 10.16774/j.cnki.issn.1674-2214.2017.02.005

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