Ａｄｖａｎｃｅｓ　ｉｎ　ｇｅｎｅｔｉｃ　ｍａｎｉｐｕｌａｔｉｏｎ　ｏｎ　Ｐｓｅｕｄｏａｌｔｅｒｏｍｏｎａｓ

ＹＡＮＧ　Ｙａｎｙａｎ，ＱＩＵ　Ｊｕａｎｐｉｎｇ，ＹＵ　Ｚｈｉｌｉａｎｇ

doi:10.16774/j.cnki.issn.1674-2214.2017.02.007

Bulletin of Fermentation Science and Technology >

2017 , Vol. 46 >Issue 2: 100 - 106

DOI: https://doi.org/10.16774/j.cnki.issn.1674-2214.2017.02.007

Ａｄｖａｎｃｅｓ　ｉｎ　ｇｅｎｅｔｉｃ　ｍａｎｉｐｕｌａｔｉｏｎ　ｏｎ　Ｐｓｅｕｄｏａｌｔｅｒｏｍｏｎａｓ

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Ｃｏｌｌｅｇｅ　ｏｆ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ　ａｎｄ　Ｂｉｏｅｎｇｉｎｅｅｒｉｎｇ，Ｚｈｅｊｉａｎｇ　Ｕｎｉｖｅｒｓｉｔｙ　ｏｆ　Ｔｅｃｈｎｏｌｏｇｙ，Ｈａｎｇｚｈｏｕ　３１００１４，Ｃｈｉｎａ

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Ｐｓｅｕｄｏａｌｔｅｒｏｍｏｎａｓ　ｗａｓ　ｕｂｉｑｕｉｔｏｕｓ　ｉｎ　ｅｎｖｉｒｏｎｍｅｎｔｓ　ａｎｄ　ｃａｎ　ｐｒｏｄｕｃｅ　ｄｉｖｅｒｓｅ　ｂｉｏａｃｔｉｖｅｃｏｍｐｏｕｎｄｓ．Ｓｏ　ｆａｒ，ｓｅｖｅｒａｌ　Ｐｓｅｕｄｏａｌｔｅｒｏｍｏｎａｓ　ｉｓｏｌａｔｅｓ　ｈａｖｅ　ｂｅｅｎ　ｓｅｑｕｅｎｃｅｄ　ａｎｄ　ｔｈｅｂｉｏｉｎｆｏｒｍａｔｉｃｓ　ａｎａｌｙｓｉｓ　ｈａｓ　ｒｅｖｅａｌｅｄ　ｔｈｅｉｒ　ａｂｉｌｉｔｉｅｓ　ｔｏ　ｐｒｏｄｕｃｅ　ｄｉｆｆｅｒｅｎｔ　ｂｉｏａｃｔｉｖｅ　ｃｏｍｐｏｕｎｄｓ．Ｈｏｗｅｖｅｒ，ｔｈｅ　ｌａｃｋ　ｏｆ　ｇｅｎｅｔｉｃ　ｍａｎｉｐｕｌａｔｉｏｎ　ｓｙｓｔｅｍ　ｏｆ　Ｐｓｅｕｄｏａｌｔｅｒｏｍｏｎａｓ　ｇｒｅａｔｌｙ　ｒｅｓｔｒｉｃｔｅｄ　ｔｈｅｉｒａｐｐｌｉｃａｔｉｏｎｓ　ａｎｄ　ｓｔｕｄｉｅｓ　ａｔ　ｍｏｌｅｃｕｌａｒ　ｌｅｖｅｌ．Ｈｅｒｅ，ｗｅ　ｄｅｓｃｒｉｂｅ　ｔｈｅ　ａｄｖａｎｃｅｓ　ｉｎ　ｇｅｎｅｔｉｃ
ｍａｎｉｐｕｌａｔｉｏｎ　ｏｎ　Ｐｓｅｕｄｏａｌｔｅｒｏｍｏｎａｓ，ｉｎｃｌｕｄｉｎｇ　ｔｈｅ　ｐｒｅｐａｒａｔｉｏｎ　ｏｆ　ｃｏｍｐｅｔｅｎｔ　ｃｅｌｌｓ，ｔｈｅ　ｓｕｃｃｅｓｓｆｕｌａｐｐｌｉｃａｔｉｏｎ　ｏｆ　ｓｈｕｔｔｌｅ　ｖｅｃｔｏｒｓ，ｓｕｉｃｉｄｅ　ｋｎｏｃｋｏｕｔ　ｖｅｃｔｏｒｓ　ａｎｄ　ｓｃｒｅｅｎｉｎｇ　ｍａｒｋｅｒｓ，ｗｈｉｃｈ　ｐｒｏｖｉｄｅ　ｔｈｅｒｅｆｅｒｅｎｃｅ　ｆｏｒ　ｔｈｅ　ｄｅｖｅｌｏｐｍｅｎｔ　ｏｆ　ｇｅｎｅｔｉｃ　ｍａｎｉｐｕｌａｔｉｏｎ　ｓｙｓｔｅｍ　ｉｎ　Ｐｓｅｕｄｏａｌｔｅｒｏｍｏｎａｓ．

Key words： Ｐｓｅｕｄｏａｌｔｅｒｏｍｏｎａｓ; ｇｅｎｅｔｉｃ　ｍａｎｉｐｕｌａｔｉｏｎ; ｃｏｎｊｕｇａｔｉｏｎ

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ＹＡＮＧ　Ｙａｎｙａｎ，ＱＩＵ　Ｊｕａｎｐｉｎｇ，ＹＵ　Ｚｈｉｌｉａｎｇ . Ａｄｖａｎｃｅｓ　ｉｎ　ｇｅｎｅｔｉｃ　ｍａｎｉｐｕｌａｔｉｏｎ　ｏｎ　Ｐｓｅｕｄｏａｌｔｅｒｏｍｏｎａｓ[J]. Bulletin of Fermentation Science and Technology, 2017 , 46(2) : 100 -106 . DOI: 10.16774/j.cnki.issn.1674-2214.2017.02.007

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