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Construction and mutagenesis of engineering Saccharomyces cerevisiae  strain producing pantothenic acid

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  • College of Biotechnology and Bioengineering,Zhejiang University of Technology,Hangzhou 310014,China

Abstract

Pantothenic acid has important physiological functions involved in sugar,fat,protein  and energy metabolism.As such,it was widely used as microbial drugs,as well as food and feed   additives.To increase the yield of pantothenic acid,the expression of enzymes responsible for
pantothenic acid synthesis in Saccharomyces cerevisiae, such as ketopantoate    hydroxymethyltransferase, 2-dehydropantoate-2-reductase, pantoate-beta-alanine ligase and   polyamine oxidase were increased.The yield of pantothenic acid reached 290μg/L after 102h   incubation in pantothenic acid-free medium.The strain BYPAN01 was further mutated by   microwave irradiation and the obtained mutant BYPAN02resulted in production of 415.7μg/L   pantothenic acid,which was 43.3%higher than that of BYPAN01and 203.2%higher than that
of wild type BY4741.This study provides a new strategy for the environment-friendly and low   cost synthesis of pantothenic acid.

Cite this article

SUN Jie,JIANG Jie,REN Zheng,WEI Chun . Construction and mutagenesis of engineering Saccharomyces cerevisiae  strain producing pantothenic acid[J]. Bulletin of Fermentation Science and Technology, 2017 , 46(3) : 134 -137+142 . DOI: 10.16774/j.cnki.issn.1674-2214.2017.03.002

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