Ｅｓｔａｂｌｉｓｈｍｅｎｔ　ｏｆ　ｎｅａｒ－ｉｎｆｒａｒｅｄ　ｐｒｅｄｉｃｔｉｏｎ　ｍｏｄｅｌ　ｏｆｃｏｎｃｅｎｔｒａｔｉｏｎ　ｉｎ　ｔｈｅ　ｉｓｏｌｅｕｃｉｎｅ　ｆｅｒｍｅｎｔａｔｉｏｎ

ＧＵＯ　Ｙｕｆｅｉ，ＳＨＩ　Ｔｕｏ，ＸＵ　Ｑｉｎｇｙａｎｇ

doi:10.16774/j.cnki.issn.1674-2214.2017.03.003

Bulletin of Fermentation Science and Technology >

2017 , Vol. 46 >Issue 3: 138 - 142

DOI: https://doi.org/10.16774/j.cnki.issn.1674-2214.2017.03.003

Ｅｓｔａｂｌｉｓｈｍｅｎｔ　ｏｆ　ｎｅａｒ－ｉｎｆｒａｒｅｄ　ｐｒｅｄｉｃｔｉｏｎ　ｍｏｄｅｌ　ｏｆｃｏｎｃｅｎｔｒａｔｉｏｎ　ｉｎ　ｔｈｅ　ｉｓｏｌｅｕｃｉｎｅ　ｆｅｒｍｅｎｔａｔｉｏｎ

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１．Ｔｉａｎｊｉｎ　Ｍｏｄｅｒｎ　Ｖｏｃａｔｉｏｎａｌ　Ｔｅｃｈｎｏｌｏｇｙ　Ｃｏｌｌｅｇｅ，Ｔｉａｎｊｉｎ　３００３５０，Ｃｈｉｎａ；
２．Ｃｏｌｌｅｇｅ　ｏｆ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，Ｔｉａｎｊｉｎ　Ｕｎｉｖｅｒｓｉｔｙ　ｏｆ　Ｓｃｉｅｎｃｅ　ａｎｄ　Ｔｅｃｈｎｏｌｏｇｙ，Ｔｉａｎｊｉｎ　３００４５７，Ｃｈｉｎａ

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Ｔｈｅ　ｎｅａｒ－ｉｎｆｒａｒｅｄ　ｓｐｅｃｔｒｕｍ　ｓｃａｎｎｉｎｇ　ａｎａｌｙｓｉｓ　ｗａｓ　ｃａｒｒｉｅｄ　ｏｕｔ　ｕｓｉｎｇ　ｎｅａｒ　ｉｎｆｒａｒｅｄａｎａｌｙｚｅｒ　ａｎｄ　ｒｅｌａｔｅｄ　ｓｏｆｔｗａｒｅ，ａｎｄ　ｔｈｅ　ｏｐｔｉｍａｌ　ｐｒｅｄｉｃｔｉｏｎ　ｍｏｄｅｌ　ｆｏｒ　ｉｓｏｌｅｕｃｉｎｅ　ｃｏｎｃｅｎｔｒａｔｉｏｎ　ｗａｓｅｓｔａｂｌｉｓｈｅｄ　ｕｓｉｎｇ　ｎｅａｒ－ｉｎｆｒａｒｅｄ　ｓｐｅｃｔｒｏｓｃｏｐｙ　ｃｏｍｂｉｎｉｎｇ　ｗｉｔｈ　ｐａｒｔｉａｌ　ｌｅａｓｔ　ｓｑｕａｒｅｓ　ｍｅｔｈｏｄ　ｕｎｄｅｒ
ｃｏｎｄｉｔｉｏｎｓ　ｏｆ　ａ　ｓｔｒａｉｇｈｔ　ｌｉｎｅ　ｍｉｎｕｓ　ａｔ　ｗａｖｅｎｕｍｂｅｒ　ｂｅｔｗｅｅｎ　７　４００ｃｍ－１　ａｎｄ　９　１００ｃｍ－１．Ｔｈｅ　ｖａｌｕｅｏｆ　ｒｏｏｔ　ｍｅａｎ　ｓｑｕａｒｅ　ｅｒｒｏｒ　ｏｆ　ｃｒｏｓｓ　ｖａｌｉｄａｔｉｏｎ（ＲＭＳＥＣＶ），ｄｅｔｅｒｍｉｎａｔｉｏｎ　ｃｏｅｆｆｉｃｉｅｎｔ（Ｒ２）ａｎｄ　ｒｅｓｉｄｕａｌｐｒｅｄｉｃｔｉｏｎ　ｅｒｒｏｒ（ＲＰＤ）ｗａｓ　１．７３ｇ／Ｌ，０．９９７ａｎｄ　３．３６，ｒｅｓｐｅｃｔｉｖｅｌｙ．Ｔｈｒｏｕｇｈ　ｅｘｔｅｒｎａｌ　ｖａｌｉｄａｔｉｏｎ，ｔｈｅｍｏｄｅｌ　ｃｏｕｌｄ　ｗｅｌｌ　ｐｒｅｄｉｃｔ　ｔｈｅ　ｃｏｎｃｅｎｔｒａｔｉｏｎ　ｏｆ　ｉｓｏｌｅｕｃｉｎｅ　ｉｎ　ｔｈｅ　ｆｅｒｍｅｎｔａｔｉｏｎ　ｐｒｏｃｅｓｓ．

Key words： ｎｅａｒ－ｉｎｆｒａｒｅｄ; ｐｒｅｄｉｃｔｉｏｎ　ｍｏｄｅｌ; ｉｓｏｌｅｕｃｉｎｅ；Ｃｏｒｙｎｅｂａｃｔｅｒｉｕｍ　ｇｌｕｔａｍｉｃｕｎ

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ＧＵＯ　Ｙｕｆｅｉ，ＳＨＩ　Ｔｕｏ，ＸＵ　Ｑｉｎｇｙａｎｇ . Ｅｓｔａｂｌｉｓｈｍｅｎｔ　ｏｆ　ｎｅａｒ－ｉｎｆｒａｒｅｄ　ｐｒｅｄｉｃｔｉｏｎ　ｍｏｄｅｌ　ｏｆｃｏｎｃｅｎｔｒａｔｉｏｎ　ｉｎ　ｔｈｅ　ｉｓｏｌｅｕｃｉｎｅ　ｆｅｒｍｅｎｔａｔｉｏｎ[J]. Bulletin of Fermentation Science and Technology, 2017 , 46(3) : 138 -142 . DOI: 10.16774/j.cnki.issn.1674-2214.2017.03.003

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