Ｃｏｎｓｔｒｕｃｔｉｏｎ　ｏｆ　ｇｅｎｅｔｉｃ　ｅｎｇｉｎｅｅｒｉｎｇ　ｓｔｒａｉｎｓ　ｆｏｒ　ｐｒｏｄｕｃｔｉｏｎ&nbsp; ｏｆ　ｓｈｉｋｉｍｉｃ　ａｃｉｄ　ｂｙ　Ｅｓｃｈｅｒｉｃｈｉａ　ｃｏｌｉ

ＨＡＮ　Ｃｈａｏ，ＣＡＩ　Ｌｉｎｇｚｈｉ，ＭＡＯ　Ｑｉａｎ，ＬＩ　Ｙａｎｊｕｎ，ＸＩＥ　Ｘｉｘｉａｎ

doi:10.16774/j.cnki.issn.1674-2214.2017.03.004

Bulletin of Fermentation Science and Technology >

2017 , Vol. 46 >Issue 3: 143 - 146+187

DOI: https://doi.org/10.16774/j.cnki.issn.1674-2214.2017.03.004

Ｃｏｎｓｔｒｕｃｔｉｏｎ　ｏｆ　ｇｅｎｅｔｉｃ　ｅｎｇｉｎｅｅｒｉｎｇ　ｓｔｒａｉｎｓ　ｆｏｒ　ｐｒｏｄｕｃｔｉｏｎｏｆ　ｓｈｉｋｉｍｉｃ　ａｃｉｄ　ｂｙ　Ｅｓｃｈｅｒｉｃｈｉａ　ｃｏｌｉ

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１．Ｎａｔｉｏｎａｌ　ａｎｄ　Ｌｏｃａｌ　Ｕｎｉｔｅｄ　Ｅｎｇｉｎｅｅｒｉｎｇ　Ｌａｂ　ｏｆ　Ｍｅｔａｂｏｌｉｃ　Ｃｏｎｔｒｏｌ　Ｆｅｒｍｅｎｔａｔｉｏｎ　Ｔｅｃｈｎｏｌｏｇｙ，Ｔｉａｎｊｉｎ　３００４５７，Ｃｈｉｎａ；
２．Ｃｏｌｌｅｇｅ　ｏｆ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，Ｔｉａｎｊｉｎ　Ｕｎｉｖｅｒｓｉｔｙ　ｏｆ　Ｓｃｉｅｎｃｅ　ａｎｄ　Ｔｅｃｈｎｏｌｏｇｙ，Ｔｉａｎｊｉｎ　３００４５７，Ｃｈｉｎａ

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Ｓｈｉｋｉｍｉｃ　ａｃｉｄ　ｉｓ　ａｎ　ｉｍｐｏｒｔａｎｔ　ｉｎｔｅｒｍｅｄｉａｔｅ　ｆｏｒ　ａｒｏｍａｔｉｃ　ａｍｉｎｏ　ａｃｉｄｓ．Ｉｔ　ｃａｎ　ｂｅ　ｕｓｅｄ　ａｓｂｕｉｌｄｉｎｇ　ｂｌｏｃｋ　ｆｏｒ　ｔｈｅ　ｃｈｅｍｉｃａｌ　ｓｙｎｔｈｅｓｉｓ　ｏｆ　ｖａｒｉｏｕｓ　ｄｒｕｇｓ．Ｉｎ　ｔｈｉｓ　ｓｔｕｄｙ，ａ　ｈｉｇｈ－ｙｉｅｌｄ　ｓｈｉｋｉｍｉｃ
ａｃｉｄ　ｐｒｏｄｕｃｉｎｇ　ｓｔｒａｉｎ　ｗａｓ　ｃｏｎｓｔｒｕｃｔｅｄ　ｔｈｒｏｕｇｈ　ｍｅｔａｂｏｌｉｃ　ｅｎｇｉｎｅｅｒｉｎｇ．Ｔｈｅ　ｇｅｎｅｓ　ａｒｏＬａｎｄ　ａｒｏＫｅｎｃｏｄｉｎｇ　ｔｈｅ　ｓｈｉｋｉｍａｔｅ　ｋｉｎａｓｅ　ｗｅｒｅ　ｋｎｏｃｋｅｄ　ｏｕｔ　ｔｏ　ｂｌｏｃｋ　ｔｈｅ　ｓｈｉｋｉｍｉｃ　ａｃｉｄ　ｃａｔａｂｏｌｉｓｍ　ａｎｄ　ｔｈｅｇｅｎｅ　ｙｄｉＢｅｎｃｏｄｉｎｇ　ｔｈｅ　ｑｕｉｎｉｃ／ｓｈｉｋｉｍａｔｅ　ｄｅｈｙｄｒｏｇｅｎａｓｅ　ｗａｓ　ｄｅｌｅｔｅｄ　ｔｏ　ｒｅｄｕｃｅ　ｔｈｅ　ｓｙｎｔｈｅｓｉｓ　ｏｆｂｙｐｒｏｄｕｃｔ．Ｔｈｅ　ａｒｏＧ，ａｒｏＥ，ａｒｏＤａｎｄ　ａｒｏＢｇｅｎｅｓ　ｅｎｃｏｄｉｎｇ　ＤＡＨＰ　ｓｙｎｔｈａｓｅ，ｓｈｉｋｉｍｉｃ　ａｃｉｄｄｅｈｙｄｒｏｇｅｎａｓｅ，ｄｅｈｙｄｒｏｑｕｉｎｉｃ　ａｃｉｄ　ｄｅｈｙｄｒａｔａｓｅ　ａｎｄ　ｄｅｈｙｄｒｏｑｕｉｎｉｃ　ａｃｉｄ　ｓｙｎｔｈａｓｅ　ｗｅｒｅｏｖｅｒｅｘｐｒｅｓｓｅｄ　ｗｉｔｈ　ｉｎｔｒｏｄｕｃｔｉｏｎ　ｏｆ　ｃｏｎｓｔｒｕｃｔｅｄ　ｏｐｅｒｏｎｓ，ｔｏ　ｓｔｒｅｎｇｔｈｅｎ　ｔｈｅ　ｓｈｉｋｉｍｉｃ　ａｃｉｄ　ｓｙｎｔｈｅｓｉｓｐａｔｈｗａｙ．Ｆｉｎａｌｌｙ，ｔｈｅ　ｏｂｔａｉｎｅｄ　Ｅ．ｃｏｌｉ　ＳＨＩＫΔａｒｏＬΔｙｄｉＢｅｎｇｉｎｅｅｒｉｎｇ　ｓｔｒａｉｎ　ｒｅｓｕｌｔｅｄ　ｉｎ　ａ　ｙｉｅｌｄ　ｏｆｓｈｉｋｉｍｉｃ　ａｃｉｄ　ｏｆ　５．５ｇ／Ｌ　ｂｙ　ｓｈａｋｅ　ｆｌａｓｋ　ｆｅｒｍｅｎｔａｔｉｏｎ．

Key words： ｈｉｋｉｍａｔｅ; ; ｓｈｉｋｉｍａｔｅ　ｋｉｎａｓｅ; ; Ｅｓｃｈｅｒｉｃｈｉａ　ｃｏｌｉ; ; ａｒｏＬ；ｙｄｉＢ

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ＨＡＮ　Ｃｈａｏ，ＣＡＩ　Ｌｉｎｇｚｈｉ，ＭＡＯ　Ｑｉａｎ，ＬＩ　Ｙａｎｊｕｎ，ＸＩＥ　Ｘｉｘｉａｎ . Ｃｏｎｓｔｒｕｃｔｉｏｎ　ｏｆ　ｇｅｎｅｔｉｃ　ｅｎｇｉｎｅｅｒｉｎｇ　ｓｔｒａｉｎｓ　ｆｏｒ　ｐｒｏｄｕｃｔｉｏｎｏｆ　ｓｈｉｋｉｍｉｃ　ａｃｉｄ　ｂｙ　Ｅｓｃｈｅｒｉｃｈｉａ　ｃｏｌｉ[J]. Bulletin of Fermentation Science and Technology, 2017 , 46(3) : 143 -146+187 . DOI: 10.16774/j.cnki.issn.1674-2214.2017.03.004

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