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Simulated gastrointestinal tract metabolism of the   isoflavones from Chickpeas

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  • 1.Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals,Zhejiang University of Technology,Hangzhou 310014,China;
    2.College of Pharmaceutical Science,Zhejiang University of Technology,Hangzhou 310014,China

Abstract

The metabolic transformation of isoflavones from chickpeas in simulated
gastrointestinal tract was investigated.The UPLC-QTOF-MS was applied to monitor the in vitro    metabolic process of chickpeas extract and its isoflavone compounds incubated in simulated gastric    juice,intestinal juice and human intestinal flora,respectively.The extract of chickpeas    unchanged during the incubation with simulated gastric juice and intestinal juice.2metabolites    were identified from ononin,4metabolites from formononetin 7-O-β-D-glucoside 6″-O-malonate,    and 4from biochanin A 7-O-β-D-glucoside 6″-O-malonate in human intestinal flora,respectively.   However,formononetin and biochanin A didnt change significantly in human intestinal flora.
The main metabolic process of isoflavone included hydrolysis,deglycosylation,dehydroxylation   and hydroxylation.The results showed that isoflavones in chickpeas were stable in simulated    gastric juice and intestinal juice,and intestinal flora played an important role in the metabolism of   isoflavones from chickpeas.

Cite this article

LI Xinhui,LIANG Xianrui . Simulated gastrointestinal tract metabolism of the   isoflavones from Chickpeas[J]. Bulletin of Fermentation Science and Technology, 2017 , 46(3) : 147 -152 . DOI: 10.16774/j.cnki.issn.1674-2214.2017.03.005

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