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研究报告

产纳豆激酶菌中抗氧化菌株的多样性分析和鉴定

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  • 1. 徐州生物工程职业技术学院生物工程系,江苏徐州221006;
    2. 德州昂立达生物技术有限公司,山东德州253000
谢昕(1981—),女,江西高安人,讲师,研究方向为微生物药物学,Email:xx822000@163.com. 通信作者:黄继翔工程师,Email:programan9527@163.com.

基金资助

江苏高校优势学科建设工程资助项目(ZYB94)

Diversity analysis and identification of strains with antioxidantive property from Bacillus sp.

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  • 1. Department of Bioengineering, Xuzhou Engineering Vocational and Technical College, Xuzhou 221006, China;
    2. Onlystar Biotechnology Co., LTD., Dezhou, 253000, China

摘要

在产纳豆激酶菌株Bacillus sp.中分离筛选具有抗氧化能力的芽孢杆菌菌株,以豆粕为原料,以抗氧化性测试为筛选体系。对筛选得到的12 个菌株的发酵上清液进行抗氧化稳定性测试,结果显示:HFBL261 菌株抗氧化力残留最高,对HFBL261 的表型形状和16S rDNA 序列进行测定,该菌株鉴定为Bacillus subtilis。

本文引用格式

谢昕,吕晓腾,黄继翔 . 产纳豆激酶菌中抗氧化菌株的多样性分析和鉴定[J]. 发酵科技通讯, 2015 , 44(1) : 12 -18 . DOI: 10.16774/j.cnki.issn.1674-2214.2015.01.002

Abstract

This paper aimed to screen and isolate Bacillus strains producing antioxidantive ingredients.  The soybean meal was used as raw material and the antioxidant assay methods were used to screen the targeted strains. The antioxidation stability test on fermentation supernatant of the twelve screened strains showed that strain HFBL261 possessed highest residual antioxidant capacity and was identified as Bacillus subtilis by phenotypic characterization and 16S rDNA sequence.
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