酪胺广泛应用于生化试剂、有机化工及医药中间体。从厂区周围土壤中筛选到一株能催化L-酪氨酸脱羧产酪胺的细菌ZQM4#，鉴定为普罗威登斯菌（Providencia Ewing）。通过碳氮源、装液量、pH 值、培养温度等条件的单因素试验和正交试验，得到该菌株的摇瓶脱羧培养条件为：牛肉浸膏5 g/L，酵母浸粉5 g/L，葡萄糖5 g/L，甘油5 g/L，磷酸氢二钾2 g/L，硫酸锰0.01 g/L，维生素VB6 0.003 g/L，温度37 °C，pH 5.5。最后进行底物投料量的条件优化后，得到如下结果：10 g/L 起始底物浓度下，3 h 能100%脱羧转化成酪胺， 之后每3 h 补加10 g/L 底物，可连续补充4 次，底物仍能100%脱羧转化成酪胺。

Tyramine is widely used in biochemical reagent, organic chemical and pharmaceutical intermediates. A strain capable of catalyzing L-Tyrosine to produce tyramine was isolated and identified as Providencia Ewing. After single-factor and orthogonal tests, the optimal culture conditions were determined as follows: beef extract 5 g/L, yeast extract 5 g/L, glucose 5 g/L, glycerine 5 g/L, K2HPO4 2 g/L, MnSO4 0.01 g/L, VB6 0.003 g/L, temperature 37 °C and pH 5.5. After optimization of the transformation conditions, 10 g/L of tyrosine was completely decarboxylation into tyramine in 3 h. The substrate was then supplemented every 3 h at a concentration of 10 g/L for 4 times, and it was found that the substrate could still be completely transformed into tyramine.