筛选得到一株产腈水解酶的基因工程菌株（ZJB12048），在优化的转化条件下（反应温度50 °C，反应体系pH 值7.0（100 mmol/L 柠檬酸/柠檬酸钠缓冲溶液），湿菌体浓度为0.02 g/mL，底物浓度为150 mmol/L），其静息细胞对2-羟基-4-甲硫基丁腈具有较高的催化活力。高效液相色谱（HPLC）、液相-质谱（LC-MS）和核磁共振（NMR）分析结果表明，反应液中的转化产物为羟基蛋氨酸（2-羟基-4-甲硫基丁酸）。

ZJB12048, a nitrilase-producing genetic engineering strain, was isolated. Under the optimized transformation conditions as followed, reaction temperature 50 ° C, initial pH value 7.0 (100 mmol/L citric acid/sodium citrate buffer solution), concentration of wet cells 0.02 g/mL and the concentration of substrates 150 mmol/L, 2 -hydroxy -4 -(methylthio) butyronitrile were transferred into methionine hydroxy analogue by the rested cells. And the obtained product was identified
as 2-hydroxy-4-(methylthio) butyric acid using HPLC, LC-MS and 1H NMR.