（S）-2-氨基丁酰胺是癫痫治疗药物左乙拉西坦的关键手性中间体。筛选获得了能够立体选择性拆分2-氨基丁酰胺合成（S）-2-氨基丁酰胺的重组酰胺酶Dt-Ami 6。考察了温度、pH、有机共溶剂及不同底物浓度对催化反应的影响。结果表明，该酶的最适催化温度为40 ℃，最适底物浓度为1 mol/L，湿菌体最适添加量为8 g/L，在去离子水中反应40 min，（S）-2-氨基丁酰胺的收率为41.7%，光学纯度达94.0%。

(S)-2-aminobutyramide is the key chiral intermediate of levetiracetam, an important drug for the treatment of epilepsy. A recombinant amidase Dt-Ami 6 was screened as the best biocatalyst for enantioselective synthesis of (S) -2 -aminobutyramide. The reaction parameters including temperature, pH, organic cosolvent and substrate concentrations were investigated. The results indicated that the optimum temperature, substrate concentration, biocatalyst loading and reaction time were 40 ℃, 1 mol/L, 8 g/L and 40 min, respectively. Under these conditions, (S)-2- aminobutyramide was obtained in 41.7% yield and 94.0% e.e..