对毕赤酵母重组表达源于植物乳杆菌的β-半乳糖苷酶的5 L 罐高密度发酵进行了研究。建立了甲醇指数补料动力学模型，并考察了不同比生长速率对β-半乳糖苷酶生产的影响。四个不同比生长速率（0.010/h，0.029/h，0.043/h 和0.058/h） 的甲醇指数补料分批发酵结果表明，比生长速率对β-半乳糖苷酶生产具有显著的影响。最大β-半乳糖苷酶产量60 U/mL 在比生长速率为0.029/h 时获得。采用指数补料分批发酵工艺的β-半乳糖苷酶产量优于常规策略下的产量，该工艺在甲醇诱导后期具有明显生产优势。

Production of recombinant β -galactosidase derived from Lactobacillus plantarum by Pichia pastoris was investigated in 5 -L bioreactor. A kinetic model of methanol feeding at the exponential rate was established and the effect of specific growth rate on the β -galactosidase production was examined. In methanol induction phase, four specific growth rates, 0.010/h, 0.029/ h, 0.043/h and 0.058/h, were investigated. The specific growth rate was found to play a significant role on the β-galactosidase production. The maximum β-galactosidase production (60 U/mL) was achieved at a specific growth rate of 0.029/h. Compared with the regular feeding strategy, the exponential feeding of methanol could afford higher β-galactosidase production, especially during
the later stage of methanol induction.