为提高非水相脂肪酶法拆分DL-薄荷醇反应的底物浓度，建立了以乙酸乙烯酯为酰基供体的无溶剂体系。实验考察了底物浓度、酶量、温度和反应时间等因素对脂肪酶Lipozyme TL IM 动力学拆分DL-薄荷醇的影响，得到最佳反应条件为DL-薄荷醇浓度2.8 mol/L，酶用量0.5 g，反应温度30 ℃，反应时间6 h。此条件下，反应转化率为22.7%，e.e.p为99.1%。脂肪酶Lipozyme TL IM 重复利用20 次，酶立体选择性基本不变，相对酶活为38.7%。

In order to enhance the substrate concentration of lipase catalyzed resolution of DLmenthol in non-aqueous phase, a solvent-free system was established using vinyl acetate as acyl donor. The effects of substrate concentration, enzyme dosage, temperature and reaction time on kinetic resolution of DL-menthol were investigated. The optimum conditions were determined as follows: substrate concentration 2.8 mol/L, enzyme dosage 0.5 g, reaction temperature 30 ℃ , reaction time 6 h. Under above conditions, the conversion reached 22.7% with e.e.p value of 99.1%. In addition, the lipase reserved 38.7% of its activity with stable enantioselectivity after 20 recycles