基于16S rDNA 序列分析和BIOLOG 快速鉴定方法鉴定杭州地区原料乳中嗜冷菌

叶慧，谢海华，黄俊，刘士旺，毛建卫，楼坚

doi:10.16774/j.cnki.issn.1674-2214.2015.04.008

发酵科技通讯 >

2015 , Vol. 44 >Issue 4: 24 - 29

DOI: https://doi.org/10.16774/j.cnki.issn.1674-2214.2015.04.008

研究报告

基于16S rDNA 序列分析和BIOLOG 快速鉴定方法鉴定杭州地区原料乳中嗜冷菌

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1. 浙江科技学院生物与化学工程学院/轻工学院，浙江杭州310023；
2. 浙江省农产品化学与生物加工技术重点实验室，浙江杭州310023；
3. 浙江省农业生物资源生化制造协同创新中心，浙江杭州310023）
4. 温州医科大学眼视光学和视觉科学国家重点实验室培育基地，浙江温州325027

叶慧（1994—），女，浙江台州人，本科生，研究方向为生物工程，E-mail：yhuiruc@163.com. 通信作者：楼坚，讲师，E-mail：loujianzust@126.com.

基金资助

国家级大学生创新创业训练计划（201311057009）

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Identification of psychrophilic bacteria in raw milk from Hangzhou area based on 16S rDNA sequences analysis and BIOLOG method

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1. School of Biological and Chemical Engineering/School of Light Industry, Zhejiang University of Science and Technology,Hangzhou 310023, China;
2. Zhejiang Provincial Key Lab for Chemical and Biological Processing Technology of Farm Products,Hangzhou 310023, China;
3. Zhejiang Provincial Collaborative Innovation Center of Agricultural Biological ResourcesBiochemical Manufacturing, Hangzhou 310023, China;
4. State Key Laboratory Cultivation Base and Key Laboratory of VisionScience, Wenzhou Medical University, Wenzhou 325027, China

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摘要

以杭州地区四个牧场的原料乳为对象，采用传统平板法分离培养嗜冷菌，得到嗜冷菌的生长曲线。通过形态学观察及镜检分离纯化出15 株形态各异的菌种。基于16S rDNA 序列分析和BIOLOG 微生物快速鉴定方法，最终鉴定为假单胞菌属、类黄假单胞菌、荧光假单胞菌、乳酸菌等种属。其中假单胞菌属有13 株，为杭州地区原料乳中嗜冷菌的优势菌。

关键词： 原料乳; 嗜冷菌; BIOLOG 鉴定; 16S rDNA 序列

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叶慧，谢海华，黄俊，刘士旺，毛建卫，楼坚 . 基于16S rDNA 序列分析和BIOLOG 快速鉴定方法鉴定杭州地区原料乳中嗜冷菌[J]. 发酵科技通讯, 2015 , 44(4) : 24 -29 . DOI: 10.16774/j.cnki.issn.1674-2214.2015.04.008

Abstract

Based on raw milk provided by four pastures in Hangzhou district, 15 psychrophile were isolated from culture plate and their growth curves were obtained. According to morphology, 16S rDNA sequences analysis and BIOLOG method, the isolated strains were identified as Pseudomonas sp., Pseudomonas synxantha, Pseudomonas fluorescens and Lactobacillus sp.. Among them, there are 13 species of Pseudomonas sp., which are the dominant bacteria in raw milk from Hangzhou.

Key words： raw milk; psychrophile microorganisms; BIOLOG identifieation; 16S rDNA sequence

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