以珍贵束丝放线菌FRCS-025 为出发菌株，采用常压室温等离子体（ARTP）诱变技术对其孢子进行处理，筛选得到了一株安丝菌素AP-3 高产菌株ARTP-054，其产量可达235.16mg/L，较原始菌株FRCS-025 的产量164.12 mg/L，提高了43.3%。以筛选出的ARTP-054 高产菌为出发菌株，进行化学诱变，选取浓度为0.4 g/L 的亚硝基胍对其孢子悬液进行处理，且使用安丝菌素AP-3 支链前体异丁醇进行底物限制性筛选，结合发酵复筛，最终筛得一株安丝菌素AP-3 高产菌株HF-064，产量达287.64 mg/L，较原始菌株产量提高了75.3%。经50 L 发酵罐验证试验，发现诱变后菌株遗传稳定性良好，168 h 产量高达292.36 mg/L。

The atmospheric pressure plasma at room temperature (ARTP) technology was applied to treat spores of Actinosynnema pretiosum FRCE-025. A high ansamitocin AP-3 producing strain ARTP-054 was obtained and the ansamitocin concentration reached 235.16 mg/L, which was increased by 43.3% compared with the original strain (164.12 mg/L). Chemical mutagenesis was further carried out on strain ARTP-054. The spores suspension was treated by 0.4 g/L nitrosoguanidine, and isobutanol, the branched chain precursor of ansamitocin AP -3, was used for substrate restrictive screening. Combined with the second-round screening, another highly producing strain HF-064 was finally screened and the yield of ansamitocin reached 287.64 mg/L, which was increased by 75.3% compared with the original strain. The 50 L fermentor test showed that the genetic stability of the mutant strain is good, and the yield of ansamitocin reached 292.36 mg/L after 168 h of fermentation.