首先研究了添加玉米浆和表面活性剂吐温对谷氨酸棒状杆菌GKGD 发酵生产α-酮戊二酸的影响． 在此基础上，通过采用添加过量玉米浆，同时在产酸期添加吐温60 的培养方法，显著提高了α-酮戊二酸的产量． 结果表明: 相比于吐温80，吐温60 更有利于α-酮戊二酸的分泌; 在初始发酵培养基中添加6 mL /L 玉米浆，在发酵10 h 加入1 mL /L 吐温60，可以使α-酮戊二酸的质量浓度达到17． 9 g /L．

In this study，the influence of corn steep liquor and surfactants addition on fermentative production of α-ketoglutarate by Corynebacterium glutamicum GKGD was investigated． The production of α-ketoglutarate was significantly improved by adding corn steep liquor in the initial fermentation medium and adding appropriate amount of Tween 60 at the beginning of acid-producing phase． The results indicated that Tween 60 was more effective in promoting the secretion of α-ketoglutarate than Tween 80． The α-ketoglutarate production achieved the highest value of 17． 9 g /L with 6 mL /L of corn steep liquor and 1 mL /L of Tween 60 added．