以色氨酸生产菌Escherichia coli TRTH 为出发菌株，在30 L 发酵罐上进行实验，在发酵10 h后随糖外源流加1 g /L 的琥珀酸，以降低代谢抑制物质的积累量，提高L-色氨酸的产量． 实验结果显示: 外源流加1 g /L 的琥珀酸发酵与未流加琥珀酸发酵相比，菌体生物量与L-色氨酸产量分别提高了5． 4%和10． 0%，乙酸积累量下降了9． 5%，糖酸转化率提高了4． 0%． 这表明外源流加1 g /L琥珀酸发酵可以提高L-色氨酸的产量，降低乙酸的积累量，提高糖酸转化率

Escherichia coli TRTH was taken as the starting strain for tryptophan production． The succinic acid was added with glucose after 10 h fermentation in the 30 L fermenter to reduce the accumulation of metabolic inhibitors and increase the L-tryptophan yield． When 1 g /L succinic acid was added，the biomass，L-tryptophan production and glucose conversion rate were increased by 5． 4%，9． 98% and 4% respectively，while the accumulation of acetic acid was decreased by 9． 5%． The results suggests that the addition of 1 g /L succinic acid can increase the production of L-tryptophan，reduce the accumulation of the acetic acid and improve the glucose conversion rate．