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研究报告

蜡样芽孢杆菌发酵产脂肪酶的分批发酵动力学

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  • 浙江工业大学药学院,浙江杭州310014
欧志敏(1973—),女,辽宁铁岭人,教授,博士,研究方向为生化制药和酶工程,E-mail:oozzmm@zjut.edu.cn.

基金资助

浙江省自然科学基金资助项目(LY15B060005)

Kinetics models of batch fermentation of Bacillus cereus#br# CGMCC No.12336for lipase production

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  • College of Pharmaceutical Science,Zhejiang University of Technology,Hangzhou 310014,China

摘要

对蜡样芽孢杆菌Bacillus cereus CGMCC No.12336发酵生产脂肪酶的动力学模型进行了研
究.分别测定了B.cereus CGMCC No.12336发酵过程中菌体细胞质量浓度、脂肪酶的活性浓度和
还原糖的质量浓度,运用Logistic,Luedeking-Piret等方程对实验数据进行了非线性拟合,阐明了
B.cereus CGMCC No.12336分批发酵过程中菌体细胞生长、产物合成和基质消耗的动力学模型
并拟合得到了动力学参数.为检验动力学模型的可靠性,对得到的模型进行了实验验证.结果表明:
动力学模型与实验数据拟合良好,能较好地描述B.cereus CGMCC No.12336分批发酵过程的动
力学特征.

本文引用格式

欧志敏,马 兰 . 蜡样芽孢杆菌发酵产脂肪酶的分批发酵动力学[J]. 发酵科技通讯, 2017 , 46(1) : 1 -5 . DOI: 10.16774/j.cnki.issn.1674-2214.2017.01.002

Abstract

The kinetics models for lipase batch fermentation by Bacillus cereus CGMCC No.12336
were studied.During the fermentation process of B.cereus CGMCC No.12336,cell density,
lipase activity and reducing sugar content were determined,respectively.Based on the Logistic
and Luedeking-Piret equation,the kinetics models of cell growth,lipase formation and substrate
consumption were established by nonlinear least-squares fitting method and the kinetic parameters
were obtained.The verification of the experimental results demonstrated the accuracy of the
model and indicated that the kinetics models were in good agreement with the experimental data,
which was used for the description of the fermentation process.
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