CNKI《中国期刊全文数据库》收录期刊
《中国核心期刊(遴选)数据库》收录期刊
《中文科技期刊数据库》收录期刊
研究报告

精氨酸对Escherichia coli发酵生产L-色氨酸的影响

展开
  • 1.代谢控制发酵技术国家地方联合工程实验室,天津300457;2.天津市氨基酸高效
    绿色制造工程实验室,天津300457;3.天津科技大学生物工程学院,天津300457
杨梦晨(1991—),男,山东青岛人,硕士研究生,研究方向为代谢控制发酵,E-mail:846756016@qq.com.

基金资助

天津市科技支撑计划重点项目(14ZCZDSY00015);天津市科技特派员项目(15JCTPJC57000)

Effect of arginine on L-tryptophan production by Escherichia coli

Expand
  • 1.National and Local United Engineering Lab of Metabolic Control Fermentation Technology,Tianjin 300457,China;
    2.Tianjin Engineering Lab of Efficient and Green Amino Acid Manufacture,Tianjin 300457,China;
    3.College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China

摘要

色氨酸是人和动物体内必须氨基酸,在食品、饲料及医药工业中具有广泛应用价值.以色氨酸
生产菌株Escherichia coli TRTH 为出发菌株,在培养基中添加精氨酸,以降低代谢副产物的积累
量,提高L-色氨酸的产量.在30L发酵罐上考察了精氨酸对L-色氨酸发酵的影响,结果表明:添加
0.2g/L的精氨酸时,菌体生物量、L-色氨酸产量和糖酸转化率分别为41.5g/L,34.5g/L和18.0%,
较未添加时分别提高了5.46%,5.83%和2.86%,且乙酸积累量较未添加时降低了10.99%.

本文引用格式

杨梦晨,刘镇瑜,陈 宁,徐庆阳 . 精氨酸对Escherichia coli发酵生产L-色氨酸的影响[J]. 发酵科技通讯, 2017 , 46(1) : 16 -19 . DOI: 10.16774/j.cnki.issn.1674-2214.2017.01.005

Abstract

L-tryptophan,one of the essential amino acids for human and animals,is widely used in
the food,feed stuff and pharmaceutical industries.L-tryptophan producing strain Escherichia
coli TRTH was used as original strain in this article and sodium arginine was added to the
medium to decrease the accumulation of by-products and increase the yield of desired product.
The effects of sodium arginine on L-tryptophan fermentation were investigated in a 30 L
fermentor.The results indicated that when added with 0.2g/L sodium arginine,the biomass,Ltryptophan
 production and glucose conversion rate were 41.5g/L,34.5g/L and 18.0%,
respectively,increased by 5.46%,5.83% and 2.86% compared with that without sodium
arginine addition,and the accumulation of acetate was decreased by 10.99%.
文章导航

/